Motif Logo Generator
Generate sequence logos for DNA or protein motifs to visualize conserved positions.
When to Use
- - Use this skill when the task is to Generate publication-quality sequence logos for DNA or protein motifs.
- Use this skill for data analysis tasks that require explicit assumptions, bounded scope, and a reproducible output format.
- Use this skill when you need a documented fallback path for missing inputs, execution errors, or partial evidence.
Key Features
- - Scope-focused workflow aligned to: Generate publication-quality sequence logos for DNA or protein motifs.
- Packaged executable path(s):
scripts/main.py. - Structured execution path designed to keep outputs consistent and reviewable.
Dependencies
See ## Prerequisites above for related details.
- -
Python: 3.10+. Repository baseline for current packaged skills. - INLINECODE4 :
unspecified. Declared in requirements.txt.
Example Usage
See ## Usage above for related details.
CODEBLOCK0
Example run plan:
- 1. Confirm the user input, output path, and any required config values.
- Edit the in-file
CONFIG block or documented parameters if the script uses fixed settings. - Run
python scripts/main.py with the validated inputs. - Review the generated output and return the final artifact with any assumptions called out.
Implementation Details
See ## Workflow above for related details.
- - Execution model: validate the request, choose the packaged workflow, and produce a bounded deliverable.
- Input controls: confirm the source files, scope limits, output format, and acceptance criteria before running any script.
- Primary implementation surface:
scripts/main.py. - Parameters to clarify first: input path, output path, scope filters, thresholds, and any domain-specific constraints.
- Output discipline: keep results reproducible, identify assumptions explicitly, and avoid undocumented side effects.
Quick Check
Use this command to verify that the packaged script entry point can be parsed before deeper execution.
CODEBLOCK1
Audit-Ready Commands
Use these concrete commands for validation. They are intentionally self-contained and avoid placeholder paths.
CODEBLOCK2
Workflow
- 1. Confirm the user objective, required inputs, and non-negotiable constraints before doing detailed work.
- Validate that the request matches the documented scope and stop early if the task would require unsupported assumptions.
- Use the packaged script path or the documented reasoning path with only the inputs that are actually available.
- Return a structured result that separates assumptions, deliverables, risks, and unresolved items.
- If execution fails or inputs are incomplete, switch to the fallback path and state exactly what blocked full completion.
Installation
CODEBLOCK3
Dependencies:
- -
logomaker - Generate publication-quality sequence logos - INLINECODE13 - Data manipulation for sequence alignment
- INLINECODE14 - Numerical operations
- INLINECODE15 - Visualization backend
Quick Start
CODEBLOCK4
Usage
Python API
CODEBLOCK5
Command Line
CODEBLOCK6
Output
Generates a sequence logo showing:
- - Letter height = information content (conservation)
- Letter stack = frequency at each position
- Y-axis: bits (information content) for DNA, or relative frequency for protein
Example
Input (FASTA):
CODEBLOCK7
Output: Logo with position 2 showing C/G variability and other positions conserved.
Risk Assessment
| Risk Indicator | Assessment | Level |
|---|
| Code Execution | Python/R scripts executed locally | Medium |
| Network Access |
No external API calls | Low |
| File System Access | Read input files, write output files | Medium |
| Instruction Tampering | Standard prompt guidelines | Low |
| Data Exposure | Output files saved to workspace | Low |
Security Checklist
- - [ ] No hardcoded credentials or API keys
- [ ] No unauthorized file system access (../)
- [ ] Output does not expose sensitive information
- [ ] Prompt injection protections in place
- [ ] Input file paths validated (no ../ traversal)
- [ ] Output directory restricted to workspace
- [ ] Script execution in sandboxed environment
- [ ] Error messages sanitized (no stack traces exposed)
- [ ] Dependencies audited
Prerequisites
CODEBLOCK8
Evaluation Criteria
Success Metrics
- - [ ] Successfully executes main functionality
- [ ] Output meets quality standards
- [ ] Handles edge cases gracefully
- [ ] Performance is acceptable
Test Cases
- 1. Basic Functionality: Standard input → Expected output
- Edge Case: Invalid input → Graceful error handling
- Performance: Large dataset → Acceptable processing time
Lifecycle Status
- - Current Stage: Draft
- Next Review Date: 2026-03-06
- Known Issues: None
- Planned Improvements:
- Performance optimization
- Additional feature support
Output Requirements
Every final response should make these items explicit when they are relevant:
- - Objective or requested deliverable
- Inputs used and assumptions introduced
- Workflow or decision path
- Core result, recommendation, or artifact
- Constraints, risks, caveats, or validation needs
- Unresolved items and next-step checks
Error Handling
- - If required inputs are missing, state exactly which fields are missing and request only the minimum additional information.
- If the task goes outside the documented scope, stop instead of guessing or silently widening the assignment.
- If
scripts/main.py fails, report the failure point, summarize what still can be completed safely, and provide a manual fallback. - Do not fabricate files, citations, data, search results, or execution outcomes.
Input Validation
This skill accepts requests that match the documented purpose of motif-logo-generator and include enough context to complete the workflow safely.
Do not continue the workflow when the request is out of scope, missing a critical input, or would require unsupported assumptions. Instead respond:
INLINECODE18 only handles its documented workflow. Please provide the missing required inputs or switch to a more suitable skill.
Response Template
Use the following fixed structure for non-trivial requests:
- 1. Objective
- Inputs Received
- Assumptions
- Workflow
- Deliverable
- Risks and Limits
- Next Checks
If the request is simple, you may compress the structure, but still keep assumptions and limits explicit when they affect correctness.
Motif Logo Generator
为DNA或蛋白质基序生成序列标识图,以可视化保守位置。
使用时机
- - 当任务需要为DNA或蛋白质基序生成可发表质量的序列标识图时,使用此技能。
- 当数据分析任务需要明确的假设、有限的范围和可重复的输出格式时,使用此技能。
- 当需要为缺失输入、执行错误或部分证据提供有文档记录的备用路径时,使用此技能。
主要特性
- - 聚焦范围的工作流程,针对:为DNA或蛋白质基序生成可发表质量的序列标识图。
- 打包的可执行路径:scripts/main.py。
- 结构化的执行路径,旨在保持输出一致且可审查。
依赖项
相关详情请参见上方的## 先决条件。
- - Python:3.10+。当前打包技能的仓库基线。
- numpy:未指定。在requirements.txt中声明。
使用示例
相关详情请参见上方的## 用法。
bash
cd 20260318/scientific-skills/Data Analytics/motif-logo-generator
python -m py_compile scripts/main.py
python scripts/main.py --help
示例运行计划:
- 1. 确认用户输入、输出路径以及任何必需的配置值。
- 如果脚本使用固定设置,编辑文件内的CONFIG块或文档化参数。
- 使用验证后的输入运行python scripts/main.py。
- 审查生成的输出,并返回最终产物,同时注明所有假设。
实现细节
相关详情请参见上方的## 工作流程。
- - 执行模型:验证请求,选择打包的工作流程,并生成有限的可交付成果。
- 输入控制:在运行任何脚本之前,确认源文件、范围限制、输出格式和验收标准。
- 主要实现面:scripts/main.py。
- 需优先明确的参数:输入路径、输出路径、范围过滤器、阈值以及任何领域特定的约束。
- 输出规范:保持结果可重复,明确标识假设,避免未文档化的副作用。
快速检查
在深入执行之前,使用此命令验证打包脚本入口点是否可解析。
bash
python -m py_compile scripts/main.py
审计就绪命令
使用这些具体命令进行验证。它们特意保持自包含,避免使用占位符路径。
bash
python -m py_compile scripts/main.py
python scripts/main.py --help
工作流程
- 1. 在进行详细工作之前,确认用户目标、必需输入和不可协商的约束。
- 验证请求是否与文档化范围匹配,如果任务需要不受支持的假设,则提前停止。
- 仅使用实际可用的输入,使用打包的脚本路径或文档化的推理路径。
- 返回结构化结果,区分假设、可交付成果、风险和未解决项。
- 如果执行失败或输入不完整,切换到备用路径,并明确说明阻止完整完成的具体原因。
安装
text
cd /Users/z04030865/.openclaw/workspace/skills/motif-logo-generator
pip install -r requirements.txt
依赖项:
- - logomaker - 生成可发表质量的序列标识图
- pandas - 序列比对的数据操作
- numpy - 数值运算
- matplotlib - 可视化后端
快速入门
text
从FASTA文件生成标识图
python scripts/main.py --input sequences.fasta --output logo.png --type dna
从原始序列生成标识图
python scripts/main.py --sequences ACGT\nACCT\nAGGT --output logo.png --type dna
蛋白质序列,自定义样式
python scripts/main.py --input proteins.fasta --output logo.pdf --type protein --title 保守结构域
用法
Python API
python
from motiflogogenerator import generate_logo
从文件
logo = generate_logo(
input_file=sequences.fasta,
seq_type=dna,
output_path=logo.png,
title=我的基序
)
从序列列表
sequences = [
ACGTAGCT,
ACGTAGCT,
ACCTAGCT,
ACGTAGTT
]
logo = generate_logo(
sequences=sequences,
seq_type=dna,
output_path=logo.png
)
命令行
text
python scripts/main.py [选项]
必需:
--input PATH 输入FASTA文件(或使用--sequences)
--sequences TEXT 原始序列,以换行符分隔(或使用--input)
--output PATH 输出文件路径(.png, .pdf, .svg)
可选:
--type {dna,protein} 序列类型(默认:dna)
--title TEXT 标识图标题
--width INT 图形宽度,单位英寸(默认:10)
--height INT 图形高度,单位英寸(默认:3)
--colorscheme TEXT 配色方案(默认:classic)
DNA:classic, base_pairing
蛋白质:chemistry, hydrophobicity, classic
输出
生成的序列标识图显示:
- - 字母高度 = 信息含量(保守性)
- 字母堆叠 = 每个位置的频率
- Y轴:DNA的比特数(信息含量),或蛋白质的相对频率
示例
输入(FASTA):
>seq1
ACGT
>seq2
ACGT
>seq3
ACCT
>seq4
AGGT
输出:标识图显示位置2存在C/G变异,其他位置保守。
风险评估
| 风险指标 | 评估 | 级别 |
|---|
| 代码执行 | Python/R脚本在本地执行 | 中 |
| 网络访问 |
无外部API调用 | 低 |
| 文件系统访问 | 读取输入文件,写入输出文件 | 中 |
| 指令篡改 | 标准提示词指南 | 低 |
| 数据暴露 | 输出文件保存到工作区 | 低 |
安全检查清单
- - [ ] 无硬编码凭据或API密钥
- [ ] 无未经授权的文件系统访问(../)
- [ ] 输出不暴露敏感信息
- [ ] 已实施提示注入防护
- [ ] 输入文件路径已验证(无../遍历)
- [ ] 输出目录限制在工作区内
- [ ] 脚本在沙盒环境中执行
- [ ] 错误消息已清理(不暴露堆栈跟踪)
- [ ] 依赖项已审计
先决条件
text
Python依赖项
pip install -r requirements.txt
评估标准
成功指标
- - [ ] 成功执行主要功能
- [ ] 输出符合质量标准
- [ ] 优雅处理边界情况
- [ ] 性能可接受
测试用例
- 1. 基本功能:标准输入 → 预期输出
- 边界情况:无效输入 → 优雅的错误处理
- 性能:大数据集 → 可接受的处理时间
生命周期状态
- - 当前阶段:草稿
- 下次审查日期:2026-03-06
- 已知问题:无
- 计划改进:
- 性能优化
- 额外功能支持
输出要求
每个最终响应在