WB Troubleshooter
Western Blot problem diagnosis and solutions.
Use Cases
- - High background troubleshooting
- No signal diagnosis
- Multiple bands resolution
- Band smearing fixes
Parameters
- -
symptom: Problem description - INLINECODE1 : Primary/Secondary
Returns
- - Systematic troubleshooting steps
- Likely causes ranked
- Solution recommendations
Example
Input: "High background"
Output: Check blocking buffer → antibody concentration → wash stringency
Risk Assessment
| Risk Indicator | Assessment | Level |
|---|
| Code Execution | Python/R scripts executed locally | Medium |
| Network Access |
No external API calls | Low |
| File System Access | Read input files, write output files | Medium |
| Instruction Tampering | Standard prompt guidelines | Low |
| Data Exposure | Output files saved to workspace | Low |
Security Checklist
- - [ ] No hardcoded credentials or API keys
- [ ] No unauthorized file system access (../)
- [ ] Output does not expose sensitive information
- [ ] Prompt injection protections in place
- [ ] Input file paths validated (no ../ traversal)
- [ ] Output directory restricted to workspace
- [ ] Script execution in sandboxed environment
- [ ] Error messages sanitized (no stack traces exposed)
- [ ] Dependencies audited
Prerequisites
No additional Python packages required.
Evaluation Criteria
Success Metrics
- - [ ] Successfully executes main functionality
- [ ] Output meets quality standards
- [ ] Handles edge cases gracefully
- [ ] Performance is acceptable
Test Cases
- 1. Basic Functionality: Standard input → Expected output
- Edge Case: Invalid input → Graceful error handling
- Performance: Large dataset → Acceptable processing time
Lifecycle Status
- - Current Stage: Draft
- Next Review Date: 2026-03-06
- Known Issues: None
- Planned Improvements:
- Performance optimization
- Additional feature support
WB Troubleshooter
Western Blot问题诊断与解决方案。
使用场景
- - 高背景问题排查
- 无信号诊断
- 多条带解析
- 条带拖尾修复
参数
- - symptom：问题描述
- antibody_type：一抗/二抗
返回结果
- - 系统性故障排查步骤
- 按可能性排序的潜在原因
- 解决方案建议
示例
输入：高背景
输出：检查封闭缓冲液 → 抗体浓度 → 洗涤严格度
风险评估
| 风险指标 | 评估 | 等级 |
|---|
| 代码执行 | 本地执行Python/R脚本 | 中 |
| 网络访问 |
无外部API调用 | 低 |
| 文件系统访问 | 读取输入文件，写入输出文件 | 中 |
| 指令篡改 | 标准提示指南 | 低 |
| 数据泄露 | 输出文件保存至工作区 | 低 |
安全检查清单
- - [ ] 无硬编码凭证或API密钥
- [ ] 无未经授权的文件系统访问（../）
- [ ] 输出不暴露敏感信息
- [ ] 已实施提示注入防护
- [ ] 输入文件路径已验证（无../遍历）
- [ ] 输出目录限制在工作区内
- [ ] 脚本在沙盒环境中执行
- [ ] 错误信息已清理（不暴露堆栈跟踪）
- [ ] 依赖项已审计
前置条件
无需额外Python包。
评估标准
成功指标
- - [ ] 成功执行主要功能
- [ ] 输出符合质量标准
- [ ] 优雅处理边缘情况
- [ ] 性能可接受
测试用例
- 1. 基本功能：标准输入 → 预期输出
- 边缘情况：无效输入 → 优雅错误处理
- 性能：大数据集 → 可接受处理时间
生命周期状态
- - 当前阶段：草稿
- 下次审核日期：2026-03-06
- 已知问题：无
- 计划改进：
- 性能优化
- 额外功能支持
